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革兰氏染色机-细菌学中广泛使用的一种鉴别染色法

作者:     来源:网络来源     发布时间:2016-08-01 21:29:47

 自动革兰氏染色仪全自动革兰氏染色仪 革兰氏染色• 革兰氏染色法是细菌学中广泛使用的一种鉴别染色法

1884年由丹麦医师Gram创立。• 未经染色之细菌由于其与周围环境折光率差别甚小

故在显微镜下极难观察。染色后细菌与环境形成鲜明对比可以清楚地观察到细菌的形态、排列及某些结构特征而用以分类鉴定。

革兰氏染色 有污染性和危险性的方法没有价值的无聊的实验程序很多手工步骤很长的过程非常需要技巧

一般的革兰氏染色机特点有:

革兰氏染色 有污染性和危险性的方法没有价值的无聊的实验程序很多手工步骤很长的过程非常需要技巧

手工革兰氏染色 PREVI Color GramIVD CE Marked全自动革兰氏染色系统 PREVI Color Gram脱色复染剂碘液结晶紫蒸馏水乙醇固件循环染色盘盖子 无交叉污染标准化染色低试剂消耗喷雾嘴PREVI Color Gram独创性的革兰氏染色 节约型容易使用标准化可调节安全 清洁快速结果生态型高通量PREVI Color Gram 优点 Easy to useStandardizedFlexibleHigh ThroughputSafe & CleanRapid ResultEcologicalPREVI Color Gram 优点节约型 节约型低试剂损耗Alcohol (Fixation) (pump E)Crystal violent* (pump C)Distilled water (pump D)Iodine* (pump B)Distilled water (pump D)Decolorizer* (pump A)Distilled water (pump D)3 mL4.5 mL66 mL4.5 mL66 mL6 mL66 mLReagent试剂每次循环的消耗量* Reagent provided by bioMerieux*These data are theoretical depend on number of slides per carrousel and the number of runs performed per day12样本载量循环染色盘每次染色循环的试剂用量平均每瓶可做90次染色循环~ 每瓶600- 1200 样本

Economical容易操作StandardizedFlexibleHigh ThroughputSafe & CleanRapid ResultEcologicalPREVI Color Gram Benefits AlcoholFixationON or OFF设置染色数目着色循环卸载玻片放入染色玻片太好了! 玻片已经干了容易操作Load and Go…乙醇固定 EconomicalEasy to useStandardized可调节High ThroughputSafe & CleanRapid ResultEcologicalPREVI Color Gram 优点 调节结晶紫调节碘液调节脱色液可根据涂抹菌液的浓度调整染色液用量可调节可手工或者自动固定4种脱色复染液体可选丙酮番红番红丙酮品红品红 EconomicalEasy to use标准化FlexibleHigh ThroughputSafe & CleanRapid ResultEcologicalPREVI Color Gram 优点 Old culture CSF, urine,, etc Vaginal, sputum, bronchialwashes, CSF, urines, Sputum, bronchialwashes, CSF, urinesSputum, bronchialwashes, tissues, blood cultures Very thick smears, stools, blood cultures, etc, Very Thin smearsThin smearsThin & medium SmearsMedium & Thick Smears

无须操作技术技巧Thick SmearsVery Thick SmearsDECOLORIZER SETTING* These are suggested settings only. Each laboratory should establish their own for gram stainingTHICKNESSSUGGESTED SPECIMEN标准化 Fully automatedEasy to useStandardizedFlexibleHigh ThroughputSafe & Clean快速结果EcologicalPREVI Color Gram

优点 快速结果(1)1234567Fixation (pump E) (optional)Crystal violent (pump C)Wash (pump D)Iodine (pump B)Wash (pump D)Decolorizer with counterstain (pump A) 28 sWash (pump D)High Speed DryTotal without Alcohol fixationTotal with Alcohol fixation70 s34 s20 s24 s20 s20 s53 s225 s298 sCycle timing for carousel of 12 slides

快速结果: 3 - 5 min 可处理 12 个样本 EconomicalEasy to useStandardizedFlexible高通量Safe & CleanRapid ResultEcologicalPREVI Color Gram 优点 载片转子容量 :快速结果 « 小于 5 min »12 样本载片30 样本载片可处理300样本 / 小时可处理120 样本 / 小时12 Slides30 Slides高通量 EconomicalEasy to useStandardizedFlexibleHigh ThroughputSafe & CleanRapid Result生态型PREVI Color Gram 优点 EconomicalEasy to useStandardizedFlexibleHigh Throughput安全 清洁Rapid ResultEcologicalPREVI Color Gram Benefits

安全 清洁脱色复染剂:F= 高易燃性R11= 刺激性R36= 对眼睛有刺激性&封闭系统废物容器无人为接触试剂有色清洁,

有色安全 转子便于观察细胞形态同步处理8个样本2种吸收衬垫厚度可选双样本仓安全可靠简单、可调节的设计.

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